Signal Transduction Reagents and Kits
Promega CDK3/CyclinE1 Kinase Enzyme System, 10microg
Recombinant full-length human CDK3 and Cyclin E1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK3/CyclinE1 is a member of the cyclin-dependent protein kinase family.
Promega PKC theta Kinase Enzyme System, 1mg
Recombinant full-length human PKC theta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein Kinase C theta (PKC theta) is an important component in the intracellular signaling cascade.
Promega ADP-Glo™ Kinase Assay + ZAK Kinase Enzyme System
Recombinant full-length human ZAK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ZAK is a member of the MAPKKK family of signal transduction molecules and mediates gamma radiation signaling leading to cell cycle arrest.
Promega NEK3 Kinase Enzyme System, 10microg
Recombinant full-length human NEK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK3 is a member of the NEK family of protein kinases that share high amino acid homology with NIMA (never in mitosis gene a).
Promega ADP-Glo™ Kinase Assay + JNK3 Kinase Enzyme System
Recombinant full-length human JNK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. JNK3 is a member of the c-Jun N-terminal kinases (JNKs), which are part of the mitogen-activated protein (MAP) kinase family.
Promega ADP-Glo™ Kinase Assay + BRK Kinase Enzyme System
Recombinant full-length human BRK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. BRK is a member of the non-receptor tyrosine kinases (PTKs) that contains an amino terminal SH3 and SH2 domain as well as the catalytic domain.
Promega CDC7/DBF4 Kinase Enzyme System, 1mg
Recombinant full-length human CDC7 and DBF4 proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ABL1 (E255K) Kinase Enzyme System, 10microg
Recombinant human ABL1 (E255K) (27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + TAK1-TAB1 Kinase Enzyme System
Recombinant human proteins TAK1 (1-303) and TAB1 (437-end), linked by a small peptide (DFGGGGG), were expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + ACK Kinase Enzyme System
ACK is a tyrosine kinase that binds CDC42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of CDC42Hs.
Promega ADP-Glo™ Kinase Assay + TGFβR1 Kinase Enzyme System
TGF beta R1 is a member of the TGF beta receptor subfamily and is a ser/thr protein kinase that forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF beta signal from the cell surface to the cytoplasm.
Promega ADP-Glo™ Kinase Assay + NUAK2 Kinase Enzyme System
Recombinant full-length human NUAK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NUAK2 or SNF1/AMP kinase-related kinase (SNARK) is a member of the NUAK family of SNF1-like kinase 2.
Promega EGFR (L858R) Kinase Enzyme System, 10microg
Recombinant human EGFR (L858R) (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity.
Promega MYO3 beta Kinase Enzyme System, 1mg
Recombinant human MYO3 beta (1-326) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MYO3 beta is a member of the Class III myosins that are actin-dependent motor proteins containing an amino-terminal kinase domain.
Promega ADP-Glo™ Kinase Assay + AMPK (A1/B1/G2) Kinase Enzyme System
Recombinant full-length human AMPK (combination of A1/B1/G2 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. AMP-activated protein kinase (AMPK) exhibits a key role as master regulator of cellular energy homeostasis.